Exploring Nonnatural Evolutionary Pathways by Saturation Mutagenesis: Rapid Improvement of Protein Function
نویسندگان
چکیده
منابع مشابه
Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis.
Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of random mutagenesis followed by comprehensive saturation mutagenesis to further impr...
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ژورنال
عنوان ژورنال: Journal of Molecular Evolution
سال: 1999
ISSN: 0022-2844
DOI: 10.1007/pl00006593